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human epithelial pca cell line du145  (ATCC)


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    ATCC human epithelial pca cell line du145
    . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells <t>(DU145),</t> in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.
    Human Epithelial Pca Cell Line Du145, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+epithelial+pca+cell+line+du145/DU+145/pmc12971273-53-0-11
    Average 99 stars, based on 8547 article reviews
    human epithelial pca cell line du145 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants"

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants

    Journal: Bio-protocol

    doi: 10.21769/BioProtoc.5614

    . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells (DU145), in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.
    Figure Legend Snippet: . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells (DU145), in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.

    Techniques Used: Activation Assay, Control, Derivative Assay, Expressing

    Related Articles

    Activation Assay:

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.

    Control:

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.

    Derivative Assay:

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.

    Expressing:

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants
    Article Snippet: Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.Human surgical explants from patients subjected to surgical intervention for prostate cancer (Gleason Group > 3) for CAFs isolation 3.. Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.. Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood Notes: DU145 cells and ECFCs can be replaced with other similar commercially available cell lines to be used as epithelial and endothelial controls, respectively.



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    human epithelial pca cell line du145  (ATCC)
    99
    ATCC human epithelial pca cell line du145
    . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells <t>(DU145),</t> in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.
    Human Epithelial Pca Cell Line Du145, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+epithelial+pca+cell+line+du145/DU+145/pmc12971273-53-0-11
    Average 99 stars, based on 1 article reviews
    human epithelial pca cell line du145 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells (DU145), in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.

    Journal: Bio-protocol

    Article Title: Non-Enzymatic Isolation of Cancer-Associated Fibroblasts From Human Prostate Tumor Explants

    doi: 10.21769/BioProtoc.5614

    Figure Lengend Snippet: . The results indicate that collagen type I (col1a1) and vimentin are expressed in both healthy and cancer-associated fibroblasts (HPFs and CAFs), confirming their mesenchymal identity. Conversely, α-smooth muscle actin (α-SMA) is detected exclusively in CAFs, reflecting their activation compared to the HPFs. As expected, CD31 is exclusively expressed in endothelial cells (ECFCs), while E-cadherin and claudin are only detected in epithelial cells (DU145), in accordance with their role in epithelial junctions. HSP90 was used as a loading control to ensure equal protein content across samples. HPF and CAF were derived from three different explants. Note that α-SMA expression differs among the three CAF samples, reflecting inter-patient biological variability.

    Article Snippet: Human epithelial PCa cell line DU145 (RRID: CVCL 0105) obtained from ATCC and routinely tested for Mycoplasma contamination using the MycoAlert Mycoplasma Detection kit (Lonza, #LOLT07710) 4.

    Techniques: Activation Assay, Control, Derivative Assay, Expressing